Comparison of two different DNA isolation methods in the detection of equine fetal cell-free DNA

dc.contributor.author	Ramsay-Stagg, Charlotte
dc.date.accessioned	2020-03-19T18:12:15Z
dc.date.available	2020-03-19T18:12:15Z
dc.date.issued	2019
dc.identifier.uri	http://hdl.handle.net/10832/2394
dc.description.abstract	Circulating equine cell free fetal DNA must be isolated and extracted in order to carry out further diagnostics such as equine fetal sex determination. This study compared two isolation methods, a more traditional Phenol-chloroform-isoamyl alcohol method with a ZYMO cfDNA Vacuum kit to conclude which protocol may obtain the highest concentration of ccffDNA whilst taking into consideration stage of gestation which must be optimised to ensure high yields.	en_US
dc.language.iso	en	en_US
dc.title	Comparison of two different DNA isolation methods in the detection of equine fetal cell-free DNA	en_US
dc.type	Thesis	en_US