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Kutyák előesett harmadik szemhéj mirigyének kórszövettani és pancytokeratin-, ill. E-cadherin alapú immunhisztokémiai vizsgálata

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MAL 2018 10 613 (3.626Mb)
Date
2018-10
Author
Dobos, András
Varsányi, Balázs
Jakab, Csaba
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Abstract
SUMMARY Background: Prolapse of the third eyelid gland is the most common primary disorder of the canine nictitating membrane. Objectives: The aim of the present histopathological, immunohistochemical study was to describe microscopic pathomorphological lesions of the parenchyma, and the interstitium of the surgically removed prolapsed canine third eyelid glands (TEG), compared to the normal ones. Materials and Methods: Five intact, and 26 surgically removed prolapsed TEG samples were separated and conserved in buffered, 8% formalin for 24 hours at room temperature, embedded in paraffin wax and further processed for sectioning (3–4 µm) and immunohistochemistry (antibodies: anti-pancytokeratin, and anti-E-cadherine). Results and Discussion: Histopathological lesions included (n = 26): 53.84% (n = 14) acute purulent parenchymatous adenitis, which was accompanied by with acute purulent ductal inflammation in 9 cases; 34.61% (n = 9) subacute-chronic lymphocytic interstitial adenitis, coupled with acinar degeneration and necrosis in 8 cases; 30.77% (n = 8) interstitial fibrosis; 3.85% (n = 1) adenoma. During the immunohistochemical investigations, the inflamed, degenerated TEG showed weaker or even loss of the pancytokeratin-, and E-cadherine-expression.
URI
http://hdl.handle.net/10832/2791
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