| dc.description.abstract | Background: Extended spectrum ß-lactamases (ESBLs) are enzymes conferring
resistance to the majority of ß-lactam antibiotics in many bacterial species,
including Escherichia coli. Bacteria harboring these enzymes could be resistant
to 3rd and 4th class cephalosporines and carbapenems, depleting the range of
compounds available for treating serious human infections.
Objectives: The authors of this study aimed to examine the ESBL production of
50 selected E. coli strains isolated between 2016 and 2022 in Hungary from cases
of porcine diarrhea.
Materials and Methods: Archived E. coli strains suspected to produce ESBL
were selected based on results. The 50 strains tested in the study originated
from 27 different farms, 28 were collected from suckling, 22 from weaned piglets.
The phenotypic characterization of the strains was achieved using Micronaut-S
ß-lactamases MIC plates.
Results and Discussion: Phenotypic characterization of selected E. coli strains
revealed the presence of ESBL production in 35 strains, two strains additionally
produced AmpC cephalosporinase, and one strain only produced AmpC. ESBL
producing strains were found on 20 of the 27 farms investigated. Many of the
ESBL producing strains originating from cases of neonatal diarrhea did not
possess any of the examined virulence factor genes, nor were they ß-hemolytic.
ESBL producing strains from post weaning diarrhea cases on the other hand were
all ß-hemolytic and had one or several virulence factor genes. ESBL producing
strains are likely widespread, although with quite low frequency in the Hungarian
swine production system, both among pathogenic and likely commensal groups
of E. coli. Continuous monitoring of ESBL producing strains is warranted, possibly
with the inclusion of ESBL selective media or other methods of identification in
routine AST, especially in case of E. coli strains isolated from suckling piglets. | en_US |
