Kutyasperma mélyhűtése, fagyasztóoldatokkal végzett összehasonlító vizsgálatok előzetes eredményei

Abstract

Background: Semen freezing in many animal species, as well as in humans, has become a successful assisted reproductive technique in recent decades. Canine semen freezing and the use of artificial insemination with frozen-thawed semen have become popular tools among breeders. Objectives: Our aim was to evaluate five freezing mediums based on post-thaw progressive motility parameters. We intended to find relationship between the motility results of fresh, chilled, and frozen-thawed semen to evaluate the prog- nostic value of the fresh semen’s progressive motility on the post-thaw quality of dog semen. Materials and Methods: Seven clinically healthy Beagle dogs were used and five semen freezing mediums (Steridyl, Triladyl, Steridyl + Equex paste, CaniRep Uppsala Equex II, CaniPro Freeze A & B) were examined in this experimental design. The 2nd fraction of the ejaculates was collected by digital manipulation. The assessment was based on volume, color, morphology, and motility (CASA system) parameters. Fifty-eight dog semen samples were frozen and evaluated in this study. Results and Discussion: The mean progressive motility values were similar among the fresh semen groups based on extenders (79.6 to 81.7%). After freezing and thawing, we observed significantly higher mean progressive motility results with CaniRep extender (66.8%) compared to Triladyl (39.6%), Steridyl + Equex paste (37.0%), and CaniPro Freeze (40.0%) (p = 0.0316). However, the difference between the CaniRep and Steridyl groups' post-thaw progressive motility para- meters was not statistically significant (66.8 vs. 48.9%). The highest progressive motility result was achieved with CaniRep freezing medium (66.8%). With this extender, 80% of the post-thaw samples were of good quality (≥50% progressive motility). The post-thaw quality of canine semen can be better predicted from the motility results of chilled semen than from the fresh semen. The motility of post-thaw samples was significantly dependent on the extender used (p = 0.0316).