A retrospective study of the expression of TNF-alpha and IL-1bet in canine granulomatous colitis

Abstract

The goal of this retrospective study is to determine the expression of the cytokines TNF-alpha and IL-1beta in the intestinal mucosa, specifically in the colonic mucosa, of canine patients diagnosed with granulomatous colitis. For the analysis, we utilized previously collected endoscopic biopsy samples from the colon, preserved as FFPE blocks, and employed the RNAscope method to quantify cytokine expression. Statistical comparisons were performed between dogs with GC and healthy controls, focusing on the cluster per area and spot per area expression of the cytokines in the colonic mucosa.