Hipofízis adenilát cikláz aktiváló polipeptid hatása nőstény egerek ciklusára és az embriófejlődésre - előzetes eredmények

Abstract

SUMMARY Background: Due to the antiapoptotic effect of pituitary adenylate cyclase activating polypeptide (PACAP) and its widespread presence in the organ system, PACAP is considered as a general cytoprotective peptide. The peptide was found in the gonads in high levels, what drew attention to that the peptide might play a central role in reproduction.objectives: The aim of the present study is to assess the effect of the endogen peptide on embryo development and quality compared with PACAP KO mice. Moreover, the authors examine the role of the PACAP to the production of progesterone and oestradiol (with radioimmunoassay) in females and to the quality of the semen in male mice.Materials and methods: PACAP-producing (wild type) and KO CD1 mice were examined through the experiments. Zygotes produced by natural mating were obtained from donor superovulated females 5-6 hours after copulation. Embryos were cultured for 96 hours in vitro and developmental state, cell number and micronuclei rate were assessed. Faeces were collected for 3 weeks from 10 mice (5 KO and 5 wild type) and oestradiol and progesterone levels were measured with radioimmunoassay. Results and discussion: Higher blastomere number was found in PACAP KO embryos than in wild ones (p = 0,0022). However, the advanced PACAP KO embry-os have significantly higher micronucleus rate compared to advanced wild type embryos (p = 0,01). Although oestradiol peak levels did not differ significantly, PACAP-KO mice showed higher concentrations as in the case of progesterone. Due to their data, further experiments will be carried out.