Tejhasznú tehenek méhgyulladása elleni probiotikus készítmény fejlesztése és vizsgálata

Abstract

Reproductive performance issues present a significant challenge for large-scale commercial dairy cattle farms in terms of veterinary medicine, farm management and economy. Postpartum uterine diseases affect almost every second dairy cow. Generally accepted treatment protocols for uterine diseases include antibiotics, non-steroid anti-inflammatory drugs and hormones. Due to the spreading resistance towards antibiotics, demand for alternative treatment and prevention options also increases nowadays. Probiotics are currently in the focus of scientific interest. Probiotics are live microorganisms that offer beneficial effects for the host organism when administered in the proper dosage. These effects are strain-specific so potential probiotics have to go through strict in vitro and in vivo examinations before they can be clinically evaluated. The aim of our research was to identify the bacterial composition of healthy vaginal microbiome in dairy cattle and to further investigate potentially probiotic isolates thus creating an intravaginal probiotic product to prevent uterine diseases in the periparturient period. Fifty-four bacterial species were successfully identified from healthy dairy cows’ vaginal microbiome. Of these, 87% were Gram-positive and 13% were Gram-negative. Five potentially probiotic isolates were selected for resistance genes and antibiotic sensitivity testing. Citotoxicity and transepithelial electrical resistance measurements were also performed on different bacterial strains.