PROSPECTS OF CULTURE AND CRYOPRESERVATION OF CANINE PREANTRAL FOLLICLES

Abstract

Ovary of the mammals contains high numbers of preantral follicles. These follicles are suitable for in vitro culture, can be cryopreserved, and provides a large repository of oocytes. Following in vitro maturation, these oocytes can be fertilized. The procedure makes the opportunity to preserve fertility in animals with unique genetics or in women before anti-cancer treatment. However, in vitro culture protocols are not standardized, and the basal media and supplements, and preservation methods, differ among laboratories and species as well. Our study aimed to analyze the characteristics of in vitro growth of canine follicles.