Kutyák heredaganatainak incidencia-, valamint E-cadherin alapú immunhisztokémiai vizsgálata Onkopatológiai tanulmány

Abstract

SUMMARY Background: The major types of testicular tumour in dogs are sex-cord stromal tumours, germ cell tumours, and mixed type of the germ cell-, and sex-cord stromal tumours. Objectives: The first goal of the present study was to describe the canine testi cular tumour incidence in Hungary, based on the histopathological investigation of the biopsy samples at the Department of Pathology. The second aim of the present work was about the E-cadherin based immunhistochemical differentia tion between Leydig- and Sertoli cell tumours. Materials and Methods: Testicle tumour samples were separated and con served in buffered, 8% formalin for 24 hours at room temperature, embedded in paraffin wax and further processed for sectioning (3-4 µm) and immunohis tochemistry (antibody: anti-E-cadherine, rabbit, polyclonal, dilution: 1:100). The number of the biopsy samples was 147. Results and Discussion: The peak rate of testicular tumours was between the age of 9 and 12 years as well as the most emphasized breeds in connec tion with testicular tumours were the German shepherd dogs, Yorkshire terriers, poodles and mixed breed dogs. The most frequently diagnosed tumour proved to be the seminomas (53,06%), followed in order by the Leydig-cell tumours (40,14%) and Sertoli-cell tumours (14,28%). Solid Leydigomas were negative for E-cadherine, and solid Sertolimas showed intense, linear, membranous E-cad herine-positivity. We detected similar result during the E-cadherine-based immunhistochemical investigation of the lipidisated, vaculisated Leydigomas, and lipidisated (steroidogenic) Sertoliomas.