A mesenchymalis őssejtek felhasználásának lehetőségei az állatorvosi kutatásokban és gyógyításban II. rész - Saját vizsgálatok

Abstract

SUMMARY In the first part of the two-part article series the authors introduced the possibility of the application of mesenchymal stem cell (MSC) in the field of human and principally veterinary medicine. In this second article they present their own results of the in vitro proliferative capacity and osteogenic differentiation potential of adipose tissue MSCs isolated from dogs of different ages. MSC cells were isolated from canine subcutaneous adipose tissue which were proliferated, characterized according to the international standard, differentiated and frozen for following experiments. Their observation revealed that MSCs from old and young donor Beagle dogs proliferated properly in vitro and they were able to show the required fibroblast- like morphology and differentiated into adipo-, chondro- and osteogenic lineages. The osteogenic differentiation of MSCs was followed by the measurement of alkaline phosphatase activity and calcium accumulation. Both of the determined parameters were higher in the case of younger donors compared to the old donor dog.