Alfa-simaizom-actin (α-SMA) pozitív kontrollok szarvasmarhaszövetekben - Immunhisztokémiai tanulmány

Abstract

SUMMARY The aim of the present study was to analyse the immunoreactivity of α-smooth muscle actin (α-SMA), smooth muscle marker on bovine intact tissues. The following tissues showed diffuse, homogenous, intense, cytoplasmic, α-SMApositivity: skin: arrector pili muscle, tunica media cells of the dermal vessels, myoepthelial cells around the apocrin glands, and ducts; lymph node (Figs 1. and 2.), haemolymph node, spleen (Figs 3. and 4.): capsule, trabecules, extra- and intraparenchymal vessels; tongue: vessels, myoepithelial cells of the intermuscular glands; oesophagus: lamina muscularis mucosae, vessels, myoepithelial cells of the glands, tunica muscularis (Figs 5. and 6.); proventricules, abomasum: lamina muscularis mucosae, vessels, tunica muscularis (Fig 7.); small intestine, large intestine: lamina muscularis mucosae, pericryptal myofibroblasts, vessels, tunica muscularis (Figs 8. and 9.); liver: perisinusoidal myofibroblasts, portal (periductal) myofibroblasts, tunica media cells of the intrahepatic vessels (Figs 10.); kidney: smooth muscle cells of the intrareanal arteries and veins, subepithelial smooth muscle cells around the pelvis renalis, myofibroblasts of the capsule (Figs 11. and 12.); urinary bladder: lamina muscularis mucosae, vessels, tunica muscularis (Figs 13. and 14.); uterus: myometrium, smooth muscle cells of the vessels (Figs 15. and 16.); ovary gland: myofibroblast cells of the theca externa layer, vessels; lung: tunica media cells of the intrapulmonary vessels, smooth muscle cells in the wall of the micro-, macrobronchus; myocardium: tunica media cells of the coronary vessels; aorta: intramural smooth muscle cells, -small vessels. The authors propose the immunohistochemicaly validated, α-SMA-positive bovine tissues for reliable external or internal positive controls in a variety of immunohistochemical pathology studies.