The effects of chicoric acid on chicken primary hepatic cell cocultures under viral RNA analog induced inflammation

Abstract

Causative agents of hepatitis-hydropericardium, runting-stunting syndrome, chicken anemia and infectious bursal disease play a major role in the poultry industry, even in vaccinated flocks. The organ damage is triggered by excessive inflammation caused by cell death and free radicals in those diseases, thus it is essential to find supplementary substances to alleviate the negative effect. Our aim of this study was to induce inflammation with a viral double-stranded RNA (dsRNA) analog polyinosinicpolycytidylic acid (poly I:C) on chicken primary hepatocyte-non-parenchymal cell co-cultures in order to investigate possible immunomodulatory effects of chicoric acid (CA) in comparison to the antioxidant and anti-inflammatory n-acetyl-cysteine (NAC). Cell cultures were treated with 50 µg/mL poly I:C and additionally with 100 and 200 µg/mL NAC or 10 and 100 µg/mL CA for 24 hours. Following treatment, metabolic activity (CCK assay), extracellular lactate dehydrogenase (LDH) activity, interleukin-6 (IL-6), interleukin-8 (IL-8) concentrations of the medium, malondialdehyde (MDA) and caspase-3 levels of the cell lysate were measured.