A Dirofilaria immitis és Dirofilaria repens mikrofiláriák fénymikroszkópos vizsgálata - 1. rész: A mikrofiláriák felismerése a különféle mintákban

Abstract

SUMMARY The authors suggest easy methods for veterinarians with small animal practice to detect the presence of microfilariae produced by Dirofilaria immitis and Dirofilaria repens nematodes and to recognize their embryonic larvae (prelarvae). The presented simple light microscopy techniques help to reveal as many subclinical infections as possible, and not only in living animal, but also in carcasses of dead ones. As the actively moving creatures in blood can be detected easier than motionless ones, the finding of the microfilariae is promoted by using such way of haemolysis that does not kill them. Instead of the traditional Knott’s method therefore, they suggest to use sodium lauryl sulphate for haemolysis and Nile blue for staining living and dead microfilariae both in order to see them better. Clotted blood of the dead animals can be investigated easily for microfilariae if a bit of clot is immersed into a dark solution of alkaline histological dye (i.e. methylene or Nile blue) then it is pressed apart on microscopic slide.