Az újra felfedezett Magyary-Kossa - 150 éve született Magyary-Kossa Gyula - a Kóssa-reakció (2. rész)

Abstract

Summary In the first part of this series, the authors described the main milestones of Gyula Magyary-Kossa’s path of life, the history of teaching veterinary pharmacology in Hungary, the foundation of the Institute of Pharmacology and the decisive role that Magyary-Kossa played in it. Part 2 presents the ‘von Kossa reaction’, a microtechnical reaction developed by him and used all over the world to detect calcium deposits and quantify mineralization in tissue sections. The authors describe the ‘von Kossa reaction’ and the studies performed by its use in detail and then give an outline of the classical histochemical methods and their present-day applications. By performing searches in different databases and collecting the citations made to the original publications of different authors and their methods, the authors confirm that the ‘von Kossa reaction’, originally described in 1901, has not sunk into oblivion up to the present day. Rather, it is still used often, like many other easy-to-use and specific, classical histochemical procedures developed around the turn of the 19th and 20th centuries. They present the comparative data of 16 classical histochemical procedures in table (designation of the staining procedure, author, year of publication, country of origin, main area of use, number of citations, and distribution of citations by year in the past 20 years).