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dc.contributor.authorKovács, Áron Botonden
dc.contributor.authorKreizinger, Zsuzsaen
dc.contributor.authorForró, Barbaraen
dc.contributor.authorGrózner, Dénesen
dc.contributor.authorMitter, Alexaen
dc.contributor.authorMarton, Szilviaen
dc.contributor.authorBali, Krisztinaen
dc.contributor.authorSawicka, Annaen
dc.contributor.authorTomczyk, Grzegorzen
dc.contributor.authorBányai, Krisztiánen
dc.contributor.authorGyuranecz, Miklósen
dc.date.accessioned2023-09-08T08:42:05Z
dc.date.available2023-09-08T08:42:05Z
dc.date.issued2023-09
dc.identifier.citationMagyar Állatorvosok Lapja 145(9), 527-534. (2023)en
dc.identifier.urihttp://hdl.handle.net/10832/3588
dc.description.abstractBackground: Mycoplasma anserisalpingitidis is a waterfowl pathogen that mainly infects geese, and can cause significant economic losses. With the advance of whole genome sequencing technologies, new methods are available for the researchers; one emerging methodology is the core genome Multi-Locus Sequence Typing (cgMLST). The core genome contains a high percentage of the coding DNA sequence (CDS) set of the tested strains. Objectives: The aim of this study was to set up a precise and robust cgMLST schema for the genotyping of M. anserisalpingitidis strains. Materials and Methods: In this study, Illumina short reads of 107 M. anserisalpingitidis strains were used along with 3 complete genomes from the NCBI database, including samples from Hungary, Poland, Vietnam, and China (110 samples overall). Draft genomes were assembled with the SPAdes software and analyzed with chewBBACA program. The threshold of the presence of CDS in the strains was set to 95%, resulting in the most accurate and robust schema. Five hundred forty CDSs constituted our cgMLST schema (representing 68.87% of the whole CDS set of M. anserisalpingitidis ATCC BAA-2147), and a Neighbor joining tree was created using the allelic profiles. Result and Discussion: The phylogenetic tree from the cgMLST schema resembled the real-life relationships of the strains. The schema allowed to differentiate between strains from different integrations and managed to group the strains by geographical origin and isolation date and even strains from the same animal.en_US
dc.language.isohuen_US
dc.titleMycoplasma anserisalpingitidis törzsek vizsgálata a maggenomot célzó multilókusz szekvenciatipizáló módszerrelen_US
dc.title.alternativeThe core genome multi-locus sequence typing of Mycoplasma anserisalpingitidisen_US
dc.typeArticleen_US
dc.identifier.doi10.56385/magyallorv.2023.09.527-534


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