Meddő kancák bakteriológiai és citológiai vizsgálata a vajdasági régióban

Abstract

SUMMARY Background: While inflammation of the endometrium (endomertitis) is among the leading reproductive disorders in mares, it is often neglected during the therapy of their infertility. Ultrasonography, as well as microbiological and cytological investigations are the most frequently applied techniques in the diagnosis of endometritis. However, in most cases a single method is not efficient and the proper diagnosis requires the simultaneous evaluation of the results of more of them.objectives: The authors’ aim was to assess the reproductive disorders of mares in the Vojvodina region, paying special attention to the endometritis incidence rate, possible causes, as well as to the applicability of the diagnostic methods available in field conditions.Materials and methods: 21 infertile mares were included in the investigation. Their uterine swabs were taken with a double protected cytobrush catheter. The obtained samples were undergone to bacteriological and cytological testing. Results and discussion: Cytological findings revealed severe inflammation in 23.8 % (n = 5) of the cases (more than 5 PMN/HPF ) and Str. equi spp. zooepidemicus was isolated from all of these samples. Moderate inflammation (2–5 PMN/HPF) was cytologically detected in 28.5% (n = 6) of the mares. In this group 9.5% (n = 2) of them were bacteriologically negative, Corynebacterium strains were isolated at the same ratio, while E. coli and Pseudomonas sp. were both found in 4.7% (n = 1). According to our investigation, simultaneous cytological and bacteriological testing of uterine samples gives more accurate information on the cause of endometritis than both methods separately. The fact that 80% of the samples were bacteriologically positive proves that inflammation in the uterus is among the main causes of infertility in mares.