| dc.description.abstract | SUMMARY
Background: The oral hygiene in dogs depends mostly on the quality of con sumed food and plaque removal performed by the owner. In neglected cases
severe, irreversible periodontitis with pockets containing anaerobic bacterial
flora could evolve in months or years.
Objectives: The aim of this study was to isolate Porphyromonas spp. and Prevotella spp. from subgingival samples taken from dogs with moderate to severe
clinical periodontal diseases, and the susceptibility testing of these strains, with
special regard to pradofloxacin.
Materials and Methods: Samples for the study were collected while the dogs
were under general anaesthesia. We collected 150 periodontal samples from 59
dogs. The samples were shipped directly to Duo-Bakt Laboratórium, Budapest,
Hungary. At Duo-Bakt Laboratórium the samples were inoculated on a Wilkins–
Chalgren (incl. 8% sheep blood) plate and on another plate, which included nalidixic acid and colistin. All plates were incubated in an anaerobic environment
at 35 °C for 5–7 days, after which all pigmented colonies and the suspected
non-pigmented Prevotella spp. colonies were selected. The identification was
performed by analysing the morphology of the colonies, pigmentation, and the
results of the presumptive tests. Final identification was performed by MALDITOF-mass spectroscopy.
Results and Discussion: During the study, 74 Porphyromonas and 2 Prevotella
isolates were isolated, identified and transported to Laboklin, Bad Kissingen,
Germany for antibacterial susceptibility testing. According to our results, pradofloxacin could be a promising choice in the treatment of periodontitis as in most
cases the MIC values were under 0.1 mg/l. Based on our clinical experiences
and the MIC values, amoxicillin-clavulanic-acid is still an appropriate antibiotic
for the treatment of periodontal diseases in dogs, however, resistant strains to
clindamycin and metronidazole were found. These antibacterial susceptibility
test results could help veterinarians to create the best protocols for the treatment of periodontitis. | en_US |
