| dc.description.abstract | Coronaviruses are highly variable RNA viruses. They are distributed worldwide and infect
humans and a variety of animals. They have caused major outbreaks globally, including SARSCoV, MERS-CoV, and SARS-CoV-2. Coronaviruses can also be detrimental to animal health
and can cause huge economic impacts in animal production. Investigating the relationship
between animal and human coronaviruses may provide valuable information for both human
and veterinary medicine.
In this research, we investigate whether antibodies of human coronavirus, SARS-CoV-2 can
cross neutralise the porcine virus, TGEV and whether there is an antibody in human blood that
can neutralise the swine virus. The serological method used to investigate the cross-reactivity
for this investigation was virus neutralisation test. TGEV is an alphacoronavirus and SARSCoV-2 is a betacoronavirus. The virus neutralisation test was evaluated with neutral red
staining. A positive result was determined by the lack of cytopathogenic effects in the cell
culture. The highest dilution of serum that prevented infectivity established the neutralising
antibody titre. The virus neutralisation test is effective to evaluate serological cross-reactivity
between the antibodies of SARS-CoV-2 and TGEV that may correlate with cross-protection
between these two viruses. | en_US |
