| dc.description.abstract | Cryopreservation of bovine embryos is a biotechnological tool crucial in preserving and
conserving genetically superior embryos. Nevertheless, the process is not without
consequences, particularly impacting the viability of embryos. This review aims to
investigate the preconditioning methods to improve survivability during cryopreservation.
Preconditioning embryos employ sublethal stress to upregulate genes promoting RNA
processing, transcription and regulating protein synthesis while downregulating genes
responsible for cell death and apoptosis. Embryos are subject to osmotic pressure changes
and oxidative stress under standard cryopreservation methods. By manipulating the osmotic
or oxidative concentrations in culture conditions, embryos initiate a stress-induced response
that augments their robustness and post-cryopreservation survivability. | en_US |
