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dc.contributor.authorKerek, Ádám
dc.contributor.authorTörök, Bence
dc.contributor.authorLaczkó, Levente
dc.contributor.authorKardos, Gábor
dc.contributor.authorBányai, Krisztián
dc.contributor.authorSomogyi, Zoltán
dc.contributor.authorKaszab, Eszter
dc.contributor.authorBali, Krisztina
dc.contributor.authorJerzsele, Ákos
dc.date.accessioned2024-09-13T19:54:30Z
dc.date.available2024-09-13T19:54:30Z
dc.date.issued2023
dc.identifier.citationKerek, Ádám, Bence Török, Levente Laczkó, Gábor Kardos, Krisztián Bányai, Zoltán Somogyi, Eszter Kaszab, Krisztina Bali, and Ákos Jerzsele. 2023. "In Vitro Microevolution and Co-Selection Assessment of Florfenicol Impact on Escherichia coli Resistance Development" Antibiotics 12, no. 12: 1728. https://doi.org/10.3390/antibiotics12121728en_US
dc.identifier.urihttp://hdl.handle.net/10832/4041
dc.descriptionKerek, Ádám, Bence Török, Levente Laczkó, Gábor Kardos, Krisztián Bányai, Zoltán Somogyi, Eszter Kaszab, Krisztina Bali, and Ákos Jerzsele. 2023. "In Vitro Microevolution and Co-Selection Assessment of Florfenicol Impact on Escherichia coli Resistance Development" Antibiotics 12, no. 12: 1728. https://doi.org/10.3390/antibiotics12121728en_US
dc.description.abstractThe issue of antimicrobial resistance is becoming an increasingly serious challenge in both human and veterinary medicine. Prudent antimicrobial use in veterinary medicine is warranted and supported by international guidelines, with the Antimicrobial Advice Ad Hoc Expert Group (AMEG) placing particular emphasis on the critically important group B antimicrobials. These antimicrobials are commonly employed, especially in the poultry and swine industry. The impact of florfenicol, a veterinary antibiotic, was studied on the resistance development of Escherichia coli. The aim of the study was to investigate the effect of the use of florfenicol on the development of phenotypic and genomic resistances, not only to the drug itself but also to other drugs. The minimum inhibitory concentrations (MICs) of the antibiotics were investigated at 1×, 10×, 100× and 1000× concentrations using the adapted Microbial Evolution and Growth Arena (MEGA-plate) method. The results demonstrate that florfenicol can select for resistance to fluoroquinolone antibiotics (167× MIC value increase) and cephalosporins (67× MIC value increase). A total of 44 antimicrobial resistance genes were identified, the majority of which were consistent across the samples. Chromosomal point mutations, including alterations in resistance-associated and regulatory genes (acrB, acrR, emrR and robA), are thought to trigger multiple drug efflux pump activations, leading to phenotypically increased resistance. The study underscores the impact of florfenicol and its role in the development of antimicrobial resistance, particularly concerning fluoroquinolone antibiotics and cephalosporins. This study is the first to report florfenicol’s dose-dependent enhancement of other antibiotics’ MICs, linked to mutations in SOS-box genes (mdtABC-tolC, emrAB-tolC and acrAB-tolC) and increased multidrug efflux pump genes. Mutations in the regulatory genes acrR, emrR and rpbA support the possibility of increased gene expression. The results are crucial for understanding antimicrobial resistance and its development, highlighting the promising potential of in vitro evolutionary and coselection studies for future research.en_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.titleIn Vitro Microevolution and Co-Selection Assessment of Florfenicol Impact on Escherichia coli Resistance Developmenten_US
dc.typeArticleen_US
dc.identifier.doi10.3390/antibiotics12121728


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