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A comparison of globulins, laboratory and refractometer measured total proteins as indirect tests to diagnosis failure of passive transfer in newborn calves

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Thesis (617.2Kb)
Date
2021
Author
Moore, Sarah
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Abstract
In this study we compared globulin concentrations, total proteins measured in a laboratory, and total proteins measured with optical refractometer, to immunoglobulin G (IgG) concentrations measured by Enzyme-Linked Immunosorbent Assay (ELISA). Linear regression was used to determine their correlation coefficient (r): globulins r = 0.91, TPLab r = 0.87, and TP-Refractometer r = 0.78, their coefficient of determination (r2 ): globulins r 2 = 0.78, TP-Lab r 2 = 0.68, and TP-Refractometer r 2 = 0.51, and using the equation of the regression line the theoretical corresponding value of each of the above measurements was determined for a IgG concentration of 8 mg/ml and 10 mg/ml: globulins = <21.5 mg/ml and <23.5 mg/ml, respectively, TP-Lab = <43.5 mg/ml and <45.5 mg/ml, respectively, and TP-Refractometer = <46.5 mg/ml and 48 mg/ml, respectively. Globulins had very good correlation with IgG levels and preformed the best in this study, followed by TP-Lab, and then TP-Refractometer.
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http://hdl.handle.net/10832/4472
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