Egyedi sejtek elektroporációs töltése Ca2+-érzékeny festékkel halló egerek corti-szervének funkcionális imaging vizsgálatához

Abstract

Impairment of hearing is the most common sensory deficit in the human population. Sensorineural hearing losses (SNHLs) are the incurable forms of this impairment at present. In this case the lesion affects the inner ear (cochlea) or the neural conductivity or processing. Invention of effective drug therapy needs deepening of our patophysiological knowlege at the cellular level. Our aim was to set up and optimize the method of loading of Ca2+ indicators by electroporation, which makes easier and more reliable the funtional imaging of cells in the organ of Corti.