A Deinococcus radiodurans Nudix fehérjéjének klónozása, expressziója és enzimaktivitásának mérése
dc.contributor.author | Mohos, Szilvia | |
dc.date.accessioned | 2014-01-07T08:29:48Z | |
dc.date.available | 2014-01-07T08:29:48Z | |
dc.date.issued | 2013 | |
dc.identifier.uri | http://hdl.handle.net/10832/901 | |
dc.description.abstract | The aim of my work was the experimental examination of the disordered regions to verify my hypothesis. For this it was necessary to produce the wild-type Nudix DR0550 protein. I cloned the DNA region coding for the enzyme, cloned it into an expression vector, purified the protein and measured the enzyme activity. I worked with the wild-type as well as with the Nudix-dN mutant which is truncated at its N-terminal, lacking the longer disordered region. | en |
dc.language.iso | hu | en |
dc.subject | Baktériumok | en |
dc.subject | DNS | en |
dc.subject | Klónozás | en |
dc.subject | Bacteria | en |
dc.subject | DNA | en |
dc.subject | cloning | en |
dc.title | A Deinococcus radiodurans Nudix fehérjéjének klónozása, expressziója és enzimaktivitásának mérése | en |
dc.type | Thesis | en |