| dc.description.abstract | Background: The most widely used method for avian in vitro gene conservation is
the cryopreservation of semen. This technique is only suitable for the preservation
of male genome; therefore, an alternative method is needed for the conservation
of female genetic material. For this purpose, cryopreservation and transplantation
of gonadal tissue at day-old age can be suitable. In domestic fowl donor-derived
progeny has been regained from the transplantation of frozen / thawed ovary. In
duck donor-derived offspring was obtained from transplantation of native ovarian
tissue, however, in geese no similar results have been published.
Objectives: For performing the ovarian transplantation, a short, 15 minutes
long anaesthesia is needed. In the literature there is very few information about
the anaesthesia of waterfowl species at day-old age. The aim of this study is to
present the challenges of the anaesthesia of day-old geese and duck.
Materials and Methods: For the anaesthesia of day-old Grey Landes and White
Hungarian geese and mulard ducks the authors first applied the protocol developed for day-old chicks (combination of ketamine 10 mg/kg and xylazine 2 mg/
kg, administered intramuscularly (im.)) but this only resulted mild sedation. Then
the dosage of the medications was raised to 15 mg/kg of ketamine and 3 mg/kg
of xylazine. For the maintenance no anaesthetic machine was used, only a piece
of cotton infused with isoflurane, administered drop by drop. Subsequently, the
combination of ketamine (15 mg/kg), xylazine (3 mg/kg) and midazolam (2 mg/kg)
was administered im., then after 10 minutes the dose was repeated, which led to
cardiac arrest probably due the cumulation of drugs in the adipose tissue. After
that both in geese and ducks part the combination was given im., then after 10
minutes the leftover intravenously (iv.).
Results and Discussion: Administration of the 70% of the combination im.
then after 10 minutes the 30% iv. resulted the required level of anaesthesia. In
case of ducks, the authors found that applying the dose only intravenously was
the most suitable technique, because then the animals only need to be injected
once. Developing the anaesthesia protocol can make it possible to perform the
ovarian transplantation in day-old waterfowl species. | en_US |
