Genetikailag értékes információt hordozó kísérleti kutyaállomány szaporítása asszisztált reprodukciós eljárással

Abstract

ÖSSZEFOGLALÁS A Semmelweis Egyetem Városmajori Klinika amerikai rókakopó fajtájú kísérleti tenyészkutya állományának szaporodásbiológiai felmérésére kérték fel a Szülészeti Tanszék munkatársait, mivel az állomány reprodukciós mutatói leromlottak. A közös munka célja az állomány létszámának növelése volt. Mivel fagyasztott spermával is dolgoztak, ezért a transcervicalis intrauterin inszeminálás (TCI) alkalmazása mellett döntöttek a szerzők. A beavatkozást a szülészeti kisállatklinika munkatársai végezték. Friss spermával történt TCI-t követően 8, míg fagyasztott spermás TCI eredményeként 6 kiskutya született. Tapasztalataik jól mutatják, hogy pontos ciklusdiagnosztika és időzítés mellett, a TCI alkalmazásával megfelelő alomszámot lehet elérni. SUMMARY Background: The Obstetrics Department received a request from the Heart and Vascular Center of Semmelweis Medical University to help them to solve the problems connected with the reproduction of their experimental foxhound dog population. Objectives: The aims of our work are: 1) to select the healthy and fertile breeding bitches and sires from the existing population, 2) to make a breeding program with fresh and cryopreserved semen based on the use of endoscope assisted transcervical insemination (TCI). In this preliminary short communication, we summarize our results obtained with TCI. Materials and methods: The tracking of the cycles of the inseminated two bitches was started when they first showed the signs of heat. The first bitch was inseminated with fresh and the second one with frozen semen. In both cases TCI was used. The MOFA Medium Fiberoptic TCI device set (endoscope and related accessories) (MOFA, USA) was used for insemination carried out by the clinicians of the obstetric small animal clinic unit. At the time of the TCI, the P4 concentration of the first bitch inseminated with fresh semen was around 5 ng/mL. The TCI was repeated two days later. The second bitch had a P4 level of 18.49 ng/mL at the first TCI with frozen semen which was also repeated the next day. The sperm was cryopreserved with Bovidyl (Minitube, Germany) freezing medium. However, before AI 1 ml of Uppsala (CaniRep HB, Sweden) medium was added to the thawed semen. Results and discussion: In both females, the ultrasound pregnancy testing confirming the pregnancy was performed on days 21 and 29 after TCI. After TCI with fresh semen 8 puppies (5 females and 3 males) were born, while after TCI with frozen-thawed semen 6 puppies (2 females and 4 males). Our preliminary results clearly show that an acceptable litter size can be achieved even after TCI with cryopreserved semen, if precise cycle diagnostics and timing of TCI are carried out. However, our experience also shows that in both cases we managed to achieve a litter size typical of large dogs, in which the use of the TCI technique may play a major role.